Prognostic significance of FOXP1 as an oncogene in hepatocellular carcinoma
- Yixin Zhang1,
- Suqin Zhang1,
- Xudong Wang2,
- Jibin Liu3,
- Liping Yang3,
- Song He4,
- Li Chen4,
- Jianfei Huang5
- 1Department of Hepatobiliary Surgery, Affiliated Tumor Hospital of Nantong University, Nantong, Jiangsu Province, China
- 2Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China
- 3Department of Laboratory Medicine, Affiliated Tumor Hospital of Nantong University, Nantong, Jiangsu Province, China
- 4Department of Pathology, Affiliated Tumor Hospital of Nantong University, Nantong, Jiangsu Province, China
- 5Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China
- Correspondence to Dr Jianfei Huang, Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226001, China;
- Accepted 16 January 2012
- Published Online First 15 March 2012
Background Forkhead Box P1 (FOXP1) has been described as both a tumour suppressor candidate and a potential oncogene. The aim of this study is to identify new prognostic biomarkers and therapeutic target structures for the diagnosis and treatment of hepatocellular carcinoma (HCC).
Methods The expression of FOXP1 mRNA in HCC was characterised using real-time PCR and 20 pairs of fresh frozen HCC tissues and corresponding non-cancerous tissues. FOXP1 protein expression in HCC was confirmed using immunohistochemistry on a tissue microarray chip. Finally, FOXP1 expression was correlated with conventional clinicopathological features of HCC and patient outcome.
Results The expression of FOXP1 mRNA and protein in HCC cells was much higher than in normal hepatic cells (Z=2.315, p=0.021 and χ2=28.071, 95% CI 0.233 to 0.480, p<0.001, individually). The comparison of clinicopathological characteristics and immunohistochemistry by χ2 test analysis showed that the high expression of FOXP1 in HCC was related to large tumour diameter (χ2=6.210, p=0.013), high serum α-fetoprotein levels (χ2=6.920, p=0.031) and later stage grouping with tumour node metastasis classification (χ2=6.714, p=0.035). Kaplan–Meier survival and Cox regression analysis showed that high FOXP1 expression (HR=2.182, 95% CI 1.146 to 4.154, p=0.018) and regional lymph node metastasis (HR=2.326, 95% CI 1.037 to 5.217, p=0.041) were independent prognosis factors.
Conclusions From this investigation the authors elucidated for the first time that the correlation of high FOXP1 expression correlates with an aggressive malignant phenotype and may constitute a novel prognostic factor for HCC. These results also support a role for FOXP1 as an oncogene in HCC.
- prognostic factor
- liver cancer
- cancer research
YZ and SZ contributed equally to this study.
Funding This study was supported by grants from the Social Development and Applied Research Projects (K2010012) of Nantong, Jiangsu Province, China, and the International Cooperation and Exchanges (2011) from the Department of Health of Jiangsu Province, China.
Competing interests None.
Ethics approval This study was conducted with the approval of the Human Research Ethics Committee of the Affiliated Tumor Hospital of Nantong University.
Provenance and peer review Not commissioned; externally peer reviewed.