Cytologic distinction between high-risk and low-risk human papillomavirus (HPV) infections in SurePath™ liquid-based cell preparations

Abstract

Aims: To investigate the correlation of various diagnostic cytologic features demonstrated in SurePath liquid-based cell preparations with the infection of high-risk human papillomavirus (HPV).

Methods: A case-control study design. 510 cases of SurePath specimens that had been tested for HPV DNA by Hybrid Capture 2 assay were retrieved and re-examined for ten cytologic features. Distribution of these features in high- and low-risk HPV types was compared and risk statistics were estimated. Effects of cervicitis on the manifestation of HPV cytologic changes were adjusted by means of logistic regression.

Results: Cytologic features of nuclear hyperchromasia, multinucleation and atypical metaplastic cells were predominantly noted in high-risk HPV infection in the category of ASCUS/LSIL. The odds ratios of these three features were 6 to 12 times higher in high-risk than low-risk HPV infection.

Conclusions: Some diagnostic cytologic features can be used as markers in Pap smear screening for assessing the types of HPV infection.