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J Clin Pathol doi:10.1136/jcp.2008.056994

Analysis of microsatellite instability in colorectal carcinoma by microfluidic-based chip electrophoresis

  1. Margarete Odenthal (m.odenthal{at}uni-koeln.de)
  1. Institute of Pathology, University of, Germany
    1. Nora Barta (norabarta{at}gmx.de)
    1. Institute of Pathology, University of, Germany
      1. Daniela Lohfink (danielalohfink{at}web.de)
      1. Institute of Pathology, University of, Germany
        1. Uta Drebber (u.drebber{at}uni-koeln.de)
        1. Institute of Pathology, University of, Germany
          1. Falko Schulze (falko.schulze{at}uk-koeln.de)
          1. Institute of Pathology, University of, Germany
            1. Hans Peter Dienes (hp.dienes{at}uk-koeln.de)
            1. Institute of Pathology, University of, Germany
              1. Stephan E Baldus (stephan.baldus{at}uni-duesseldorf.de)
              1. Institute of Pathology, University of Duesseldorf, Germany
                • Published Online First 19 July 2008

                Abstract

                Microsatellite analysis is an important tool in clinical research and molecular diagnostics, because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10-15% of colorectal, gastric or endometrial carcinomas are associated with MSI, which has an impact on clinical prognosis.

                The microsatellite loci Bat25, Bat26, D2S123, D5S346, and D17S250, recommended by the Bethesda guidelines, were analysed by microfluidic-based on-chip electrophoresis in 40 cases of colon carcinoma with known MSI status. In all cases, microfluidic separation of the PCR amplicons resulted in highly resolved, distinct patterns of each of the five microsatellite loci. Detection of MSI could be demonstrated by microsatellite loci-associated, well-defined deviations in the electropherogram profiles of tumour and non-tumour material and confirmed the classification of MSI cases performed by conventional technology.

                In conclusion, microfluidic chip technology is a simple and reliable approach for MSI detection, which allows label-free and very fast analysis of microsatellite amplicons.

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