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J Clin Pathol doi:10.1136/jcp.2006.037093

The role of a recombinant hybrid protein based ELISA for the serodiagnosis of Onchocerca volvulus

  1. Julie Alison Andrews (julie.andrews{at}whittington.nhs.uk)
  1. Hospital for Tropical Diseases, United Kingdom
    1. John Bligh (john.bligh{at}uclh.org)
    1. Hospital for Tropical Diseases, United Kingdom
      1. Peter Chiodini (peter.chiodini{at}uclh.org)
      1. Hospital for Tropical Diseases, United Kingdom
        1. Jan Bradley (jan.bradley{at}nottingham.ac.uk)
        1. University of Nottingham, United Kingdom
          1. Pius Nde (piusnde{at}hotmail.com)
          1. piusnde@hotmail.com, Germany
            1. Richard Lucius (richard.lucius{at}rz.hu-berlin.de)
            1. Humboldt University, Germany
              • Published Online First 8 October 2007

              Abstract

              Onchocerca volvulus infection is traditionally diagnosed by examination of skin snips for the presence of microfilariae. A disadvantage of this method is the low sensitivity particularly with light or prepatent infection. Serodiagnosis using recombinant antigen based assays may provide a more sensitive diagnostic test. An ELISA based on a recombinant antigen OvH3 has previously been validated using sera from endemic areas. This study demonstrates its potential role as a sensitive and specific assay for use in the serodiagnosis of onchocerciasis in a reference laboratory dealing with sera from patients in non-endemic setting.

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