Table 1 Sequence of sectioning and staining for sentinel lymph node for melanoma
| Section | Treatment |
| Section 1 (first full section) | H&E |
| Section 2 | S100 |
| Section 3 | Spare |
| 50 μm gap (+50 μm) | |
| Section 4 | H&E |
| Section 5 | S100 |
| Section 6 | Spare |
| Section 7 | Spare |
| Section 8 | Spare |
| Section 9 | Spare |
| 50 μm gap (+100 μm) | |
| Section 10 | H&E |
| Section 11 | S100 |
| Section 12 | Spare |
| 50 μm gap (+150 μm) | |
| Section 13 | H&E |
| Section 14 | S100 |
| Section 15 | Spare |
| 50 μm gap (+200 μm) | |
| Section 16 | H&E |
| Section 17 | S100 |
| Section 18 | Spare |
| 50 μm gap (+250 μm) | |
| Section 19 | H&E |
| Section 20 | S100 |
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In large or round lymph nodes, the 50 μm gap may be increased up to a maximum of 100 μm.
