Integration of human papillomavirus type-16 and type-18 is a very early event in cervical carcinogenesis

Huang, L-W; Chao, S-L; Lee, B-H

doi:10.1136/jcp.2007.052027

Integration of human papillomavirus type-16 and type-18 is a very early event in cervical carcinogenesis

¹
Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
²
School of Medicine, Fu Jen Catholic University, Hsinchuang, Taipei Hsien, Taiwan
³
School of Medicine, Taipei Medical University, Taipei, Taiwan
⁴
Department of Chinese Medicine, Renai Branch, Taipei City Hospital, Taipei, Taiwan
⁵
King Car Food Industrial Co., Ltd, Yuan Shan Research Institute, Ilan, Taiwan

Lee-Wen Huang, MD, Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, 95 Wen Chang Road, Shih-Lin District, Taipei 111, Taiwan; m002057{at}ms.skh.org.tw

Accepted 16 November 2007
Published Online First 12 December 2007

Abstract

Aim: Human papillomavirus (HPV) integration is a critical event in cervical carcinogenesis. The aim of this study was to explore the physical status of HPV-16 and HPV-18 during the progression of cervical precancerous lesions.

Methods: A series of 101 HPV-16 or HPV-18 positive cervical neoplasms (32 cervical intraepithelial neoplasia (CIN) cases and 69 cervical carcinoma (CC) cases) were evaluated. The physical status of both types of HPV was assessed from paraffin-embedded formaldehyde-fixed surgical specimens by real-time PCR.

Results: For HPV-16, integrated DNA was observed in 5 (83.3%) of 6 CIN I cases, 10 (90.9%) of 11 CIN II/III cases, 29 (82.9%) of 35 FIGO (International Federation of Gynecology and Obstetrics) stage I CC cases and 16 (94.1%) of 17 FIGO stages II∼IV CC cases. For HPV-18, integrated DNA was observed in 3 (50%) of 6 CIN I cases, 5 (55.6%) of 9 CIN II/III cases, 9 (64.3%) of 14 FIGO stage I CC cases, and 1 (33.3%) of 3 FIGO stages II∼IV CC cases. The mixed form of HPV DNA was the most prevalent physical state in HPV-16. There was no significant difference between the physical state of HPV-16 and HPV-18 DNA with regard to the various grades of cervical lesions.

Conclusions: These data imply that integration of HPV-16 and HPV-18 DNA into the host genome occurs in the very early stage of cervical neoplastic progression. These early events may play an initiating role in the malignant transformation of HPV-16- and HPV-18-related low-grade lesions into high-grade dysplasia and invasive carcinoma.