Table 1 Major clinical studies of the prognostic value of disseminated tumour cells (DTC) detection in bone marrow (BM) by immunocytochemistry
and prognostic value on disease and overall survival (univariate and multivariate analysis)
| Reference | Sampling | Marker | No. patients | Detection rate (%) | Follow-up (mth) | Disease free survival | Overall survival | ||
| Univ | Multiv | Univ | Multiv | ||||||
| Redding 198379 | Smear | MUC | 110 | 28 | NA | NA | NA | NA | |
| Manegold 198880 | Biopsy | CK/PKK1 | 50 | 8 | NA | NA | NA | NA | |
| Smear | |||||||||
| Landys 199881 | Biopsy | CK/AE1–AE3, KL1, CAM 5-2 | 128 | 19 | 240 | NA | NA | Yes | NA |
| Salvadori 199082 | Biopsy | CK/MBr1 | 121 | 16.5 | 48 | No | No | NA | NA |
| Mathieu 199083 | Biopsy | MUC/EMA, HMFG2 | 93 | 1 | No | No | No | No | |
| CK/KL1, AE1–AE3, CAM5-2 | |||||||||
| Kirk 199084 | Smear | MUC/anti-milk fat globulin LICR.LON.M8.4 | 25 | 48 | 34 | No | NA | NA | NA |
| Singletary 199185 | Smear | CK/AE1, AE3, MAK-6 | 71 | 38 | 11 | No | No | No | No |
| MUC/113F1, 260F9, 317G5 | |||||||||
| Cote 199186 | Smear | MUC/C26, T16 | 49 | 36.7 | 30 | Yes | Yes | NA | Na |
| CK/AE-1 | |||||||||
| Schlimok 199287 | Cytospin | CK18/CK2 | 187 | 18 | 39 | Yes | Yes | NA | NA |
| Harbeck 199488 | Smear | CK | 100 | 38 | 34 | Yes | Yes | No | Yes |
| MUC/EMA | |||||||||
| Ménard 199489 | Cytospin | CK/MBr1, MBr8, CK18/CK2, MUC1 | 197 | 31 | NA | NA | NA | NA | NA |
| Molino 199790 | Cytospin | CK/MBr1, MBr8, MOV8, MOV16 MluC1 | 109 | 31 | 36 | No | No | No | No |
| Funke 199691 | Cytospin | CK18/CK2 | 234 | 38 | NA | NA | NA | NA | NA |
| Diel 199692 93 | Smear | MUC/TAG12 (2E11) | 727 | 43.3 | 78 | Yes | Yes | Yes | Yes |
| Mansi 199912 94 | Smear | EMA | 350 | 25.4 | 150 | Yes | No | Yes | No |
| Lyda 200095 | Biopsy | CK/AE1–AE3, 35βH11 CAM 5-2 | 54 | 31 | 38 | Yes | NA | NA | NA |
| Untch 199996 | Cytospin | CK18/CK2 | 581 | 28 | No | No | No | No | |
| Braun 200011 | Cytospin | CK/CK8,18,19 (A45 B/B3) | 552 | 36 | 36 | Yes | Yes | Yes | Yes |
| Gerber 200120 | Cytospin | CK/CK8,18,19 (5D3) | 554 | 37 | 54 | Yes | Yes | Yes | Yes |
| Gebauer200121 | Smear | CK, MUC/EMA | 396 | 42 | 75 | Yes | Yes | Yes | Yes |
| Kasimir-Bauer 200197 | Cytospin | CK/CK8,18,19 (A45 B/B3) | 128 | 34 | 24 | NA | NA | NA | NA |
| Naume 20045 | Cytospin | CK/AE1/AE3 | 819 | 13 | 49 | Yes | Yes | Yes | Yes |
| Braun 20051 | Various | Various | 4703 | 30.6 | 63 | Yes | Yes | Yes | Yes |
| Bidard 20078 | Cytospin | CK/CK8,18,19 (A45 B/B3) | 621 | 15 | 50 | Yes | Yes | Yes | Yes |
-
CK, cytokeratin; Muc, mucin; EMA, epithelial membrane antigen; NA, not available.
