COX-2 expression in ampullary carcinoma: correlation with angiogenesis process and clinicopathological variables
- G Perrone1,
- D Santini2,
- A Verzì1,
- B Vincenzi2,
- D Borzomati3,
- F Vecchio4,
- R Coppola3,
- A Antinori5,
- P Magistrelli5,
- G Tonini2,
- C Rabitti1
- 1Surgical Pathology, Campus Bio-Medico University, Rome, Italy
- 2Oncology Unit, Campus Bio-Medico University, Rome, Italy
- 3General Surgery Department, Campus Bio-Medico University, Rome, Italy
- 4Anatomic Pathology And Histology, Catholic University of Sacred Heart, Rome, Italy
- 5General Surgery Department, Catholic University of the Sacred Heart, Rome, Italy
- Correspondence to: Dr Giuseppe Perrone Surgical Pathology, Campus Bio-Medico University, Via Emilio Longoni 83, 00155 Rome, Italy; g.perrone{at}unicampus.it
- Accepted 27 September 2005
- Published Online First 17 February 2006
Abstract
Background: There is evidence that the anti-neoplastic effect of non-steroidal anti-inflammatory drugs is attributable to cyclooxygenase-2 (COX-2) inhibition, but the exact mechanisms whereby COX-2 can promote tumour cell growth remain unclear. One hypothesis is the stimulation of tumour angiogenesis by the products of COX-2 activity. To data, there have been few clinicopathological studies on COX-2 expression in human ampullary carcinoma and no data have been reported about its relation with tumour angiogenesis.
Objective: To investigate by immunohistochemistry the expression of COX-2 and the angiogenesis process in a series of primary untreated ampullary carcinomas.
Methods: Tissue samples from 40 archival ampullary carcinomas were analysed for COX-2, vascular endothelial growth factor (VEGF), and an endothelial cell marker von Willebrand factor (vWF) by immunohistochemistry, using specific antibodies.
Results: COX-2 expression was detected in 39 tissue samples (97.5%), of which two (5%) were graded as weak, 26 (65%) as moderate, and 11 (27.5%) as strong. Only one lesion (2.5%) was negative for COX-2 expression. VEGF expression was detected in 36 tissue samples (90%). A significant positive correlation was found between COX-2 and VEGF expression. No statistic correlation was found between COX-2 expression and microvessel density.
Conclusions: COX-2 is highly expressed in ampullary carcinomas. This suggests an involvement of the COX-2 pathway in ampullary tumour associated angiogenesis, providing a rationale for targeting COX-2 in the treatment of ampullary cancer.
- COX-2, cyclooxygenase-2
- HIF, hypoxia inducible factor
- HIS, immunohistochemical score
- IQR, interquartile range
- MVD, microvessel density
- PGE2, prostaglandin E2
- VEGF, vascular endothelial growth factor
- vWF, von Willebrand factor
