rss
J Clin Pathol 2006;59:211-215 doi:10.1136/jcp.2004.025452
  • Original article

Analysis of G(-174)C IL-6 polymorphism and plasma concentrations of inflammatory markers in patients with type 2 diabetes and peripheral arterial disease

  1. M Libra1,
  2. S S Signorelli2,
  3. Y Bevelacqua1,
  4. P M Navolanic1,
  5. V Bevelacqua1,
  6. J Polesel3,
  7. R Talamini3,
  8. F Stivala1,
  9. M C Mazzarino1,
  10. G Malaponte1
  1. 1Department of Biomedical Sciences, University of Catania, Via Androne, 83-95124, Catania, Italy
  2. 2Department of Internal Medicine, Angiology Unit, University of Catania
  3. 3Epidemiology Unit, Centro di Riferimento Oncologico, 33081 Aviano, Italy
  1. Correspondence to:
 Dr G Malaponte
 Department of Biomedical Sciences, University of Catania, Via Androne, 83-95124 Catania, Italy; g.malaponte{at}unict.it
  • Accepted 25 May 2005

Abstract

Aims: To determine whether the G(−174)C interleukin 6 (IL-6) polymorphism influences the development of peripheral arterial disease (PAD) in individuals with type 2 diabetes. This was investigated by comparing the distribution of G(−174)C genotypes between patients with type 2 diabetes and PAD (PAD+) and those with type 2 diabetes but without PAD (PAD−). Plasma concentrations of IL-6, fibrinogen, C reactive protein (CRP), and vascular endothelial growth factor (VEGF) were also compared in PAD+ and PAD− patients.

Methods: Blood samples were collected from 146 PAD+ and 144 PAD− patients. SfaNI was used to determine the G(−174)C genotype. Plasma concentrations of IL-6, fibrinogen, CRP, and VEGF were measured by an enzyme linked immunosorbent assay.

Results: The GG genotype was more common in PAD+ patients than in PAD− patients. PAD+ patients also had increased mean plasma concentrations of IL-6, fibrinogen, CRP, and VEGF compared with PAD− patients. Mean plasma concentrations of IL-6, fibrinogen, and CRP in both PAD+ and PAD− patients were higher in those with the GG genotype than in those with the GC or CC genotypes. In contrast, mean plasma concentrations of VEGF in PAD+ and PAD− patients were not significantly different between those with different G(−174)C genotypes.

Conclusions: These results support a model in which the GG genotype promotes PAD development among individuals with type 2 diabetes by inducing increased release of IL-6. Higher concentrations of IL-6 among those with the GG genotype is associated with increased plasma concentrations of fibrinogen and CRP.

Footnotes

  • The first two authors contributed equally to this work.

This Article

  1. Abstract
  2. Full text
  3. PDF

Responses

  1. Submit a response
  2. No responses published

Register for free content

The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.

Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.