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J Clin Pathol 58:839-844 doi:10.1136/jcp.2004.023853
  • Original article

Discordant quantitative detection of putative biomarkers in nodal micrometastases of colorectal cancer: biological and clinical implications

Table 2

 Absolute expression of CEA, CK20, and GCC mRNA detected in Dukes’ B and C colorectal cancer by R-PCR, and the presence (in bold) or absence of cancer specific expression based on the cutoff values defined in the text

Patient Stage CEA CK20 GCC
FT PET FT PET FT PET
–, no gene amplification; FT, frozen tissue; NA, not available; PET, paraffin wax embedded tissue.
1 B 3.31×1011 4.82×109 7.39×107 8.90×107
2 B 8.03×1011 6.07×109 2.56×107 1.70×106
3 B NA 1.25×109 NA NA
4 B NA 8.27×109 NA 1.99×105 NA
5 B 2.93×1011 1.83×109 3.32×108
6 B 1.34×1010 1.47×109 7.16×109
7 B 2.34×1013 1.72×1010 2.77×108 5.97×107 1.39×1011 8.64×109
8 B 2.19×109 2.13×109 1.09×109
9 B 5.59×109 2.17×108
10 B 1.88×109 2.10×107
11 B 1.09×1013 1.36×109 2.94×108 1.10×1010
12 C 3.54×1012 1.57×1012 6.28×108 5.48×107 4.35×1011
13 C 1.61×1013 8.38×1011 1.87×107 2.07×108 1.53×1012 8.47×107
14 C 9.88×1013 1.26×109 2.43×107 7.53×107
15 C 1.13×1012 1.42×1011 7.56×108 2.24×108
16 C 1.20×1015 6.30×1010 2.95×107 2.42×107 7.51×1012 1.88×107
17 C NA 3.60×1010 NA 3.84×103 NA 2.92×1010
18 C 9.76×1012 2.89×1011 1.65×108 9.69×107 4.60×1011 6.62×1010
19 C 2.14×1013 8.14×1010 4.14×109 7.90×1011 1.07×109
20 C NA 2.64×109 NA 7.54×107 NA
21 C 1.08×1011 3.87×1010 3.88×105 6.17×107 3.57×109 5.83×1010
22 C 1.65×1011 1.36×1012 7.36×108 1.30×1010 2.27×109
23 C 4.11×1011 2.24×109 7.23×105 8.89×108
24 C 2.76×1012 5.86×1011 2.01×102 8.34×109 1.93×109
25 C 2.90×1012 4.11×109 5.62×107 4.72×107 1.27×1010
26 C 2.19×1010 4.29×109 2.11×108 1.09×1010

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