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J Clin Pathol 58:710-714 doi:10.1136/jcp.2004.023424
  • Original article

HER2 amplification status in breast cancer: a comparison between immunohistochemical staining and fluorescence in situ hybridisation using manual and automated quantitative image analysis scoring techniques

Table 2

 Discrepancies seen when scoring the HercepTest by eye or using the MDS system

Sample HercepTest score FISH result Comments
By eye MDS
FISH, fluorescence in situ hybridisation; MDS, Micrometastasis Detection System.
a 2 3 Amplified Would analyse by FISH routinely but MDS accurate
b 2 1 Non-amplified MDS accurate
c 0 1 Amplified 2 tumour populations seen: 1 amplified
d 3 2 Non-amplified MDS accurate
e 2 3 Amplified Would analyse by FISH routinely but MDS accurate
f 2 1 Non-amplified MDS accurate
g 2 1 Non-amplified MDS accurate
h 2 1 Non-amplified MDS accurate
i 3 3 Non-amplified HercepTest staining inaccurate
j 3 3 Non-amplified HercepTest staining inaccurate
k 2 1 Non-amplified MDS accurate
l 2 1 Non-amplified MDS accurate
m 2 1 Non-amplified MDS accurate
n 2 1 Amplified MDS does not correspond to FISH
o 1 2 Non-amplified MDS accurate
p 2 1 Non-amplified MDS accurate
q 2 3 Amplified Would analyse by FISH routinely but MDS accurate
r 2 3 Amplified Would analyse by FISH routinely but MDS accurate
s 1 2 Amplified MDS accurate. Would not analyse by FISH routinely
t 2 1 Non-amplified MDS accurate
u 1 3 Amplified 2 tumour populations seen: 1 amplified

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