Molecular confirmation of invasive infection caused by Chaetomium globosum
- P J Paterson1,
- S Seaton1,
- T Yeghen1,
- T D McHugh1,
- J McLaughlin2,
- A V Hoffbrand3,
- C C Kibbler4
- 1Department of Microbiology, Royal Free Hospital, Pond Street, London NW3 2QG, UK
- 2Department of Histopathology, Royal Free Hospital
- 3Department of Haematology, Royal Free Hospital
- 4Department of Microbiology, Royal Free Hospital; kibbler@rfc.ucl.ac.uk
Identification of the causative organism in invasive fungal infections is difficult. Accurate and reliable diagnostic methods are required in light of the increasing incidence of emerging fungal infections.1 We recently described a method for speciating fungi in formalin fixed, paraffin wax embedded tissue sections.2 DNA is extracted using a modification of the protocol for the TaKaRa DEXPAT™ kit (TaKaRa Biomedicals, Cambrex Biosciences, Wokingham, UK), with additional steps using lyticase and ethanol precipitation.2 Amplification of the DNA is performed using panfungal primers and a method based on that described by Einsele et al.3 Identification to species level can be achieved by Southern hybridisation with a probe that binds Aspergillus fumigatus, …
