Table 2
Sequences of primers, probes, and calibration oligonucleotide
| Primer/probe | Sequence |
|---|---|
| *6-Carboxyfluorescein; †6-carboxytetramethylrhodamine. | |
| SARS-CoV | |
| Primers | |
| Forward | 5′-AGCTAACGAGTGTGCGCAAGTA-3′ |
| Reverse | 5′-TGATGTTCCACCTGGTTTAACATATAGT-3′ |
| Dual labelled fluorescent probe | 5′-(FAM*)AGCCGCCACACATGACCATCTCACTTA(TAMRA†)-3′ |
| Oligonucleotide for calibration | 5′-AGGTTAGCTAACGAGTGTGCGCAAGTATTAAGTG AGATGGTCATGTGTGGCGGCTCACTATATGTTAAACCAGGT GGAACATCATCCGG-3′ |
| GAPDH mRNA | |
| Primers | |
| Forward | 5′-GAAGGTGAAGGTCGGAGT-3′ |
| Reverse | 5′-GAAGATGGTGATGGGATTTC-3′ |
| Dual labelled fluorescent probe | 5′-(FAM*)CAAGCTTCCCGTTCTCAGCC(TAMRA†)-3′ |
