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J Clin Pathol 58:1057-1063 doi:10.1136/jcp.2004.024828
  • Original article

Augmented expression of secondary lymphoid tissue chemokine and EBI1 ligand chemokine in Crohn’s disease

Table 3

 Primers and probes used in real time quantitative polymerase chain reaction

Primer/probe Sequence
SLC
Sense primer 5′-ACCAAGCTTAGGCTGCTCCAT-3′
Antisense primer 5′-TGCACATAGCTCTGCCTGAGAG-3′
Probe (FAM labelled) 5′-AGCTATCCTGTTCTTGCCCCGCAA-3′
ECL
Sense primer 5′-AATGATGCTGAAGACTGCTGC-3′
Antisense primer 5′-TGGAAGTTCCTCACGATGTACC-3′
Probe (FAM labelled) 5′-TGTCTGTGACCCAGAAACCCATCCCT-3′
CCR7
Sense primer 5′-AAGAAGGACGTGCGGAACTT-3′
Antisense primer 5′-GGCCCACGAAACAAATGA-3′
Probe (FAM labelled) 5′-AAAGCCTGGTTCCTCCCTATCATGTACTCC-3′

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