(A) Giemsa staining: single Reed-Sternberg cell detectable within a medium sized cell population. (B) Immunostaining for CD30 (alkaline phosphatase–anti-alkaline phosphatase (APAAP); Hodgkin and Reed-Sternberg (HRS) cells and a small part of the medium sized population clearly express CD30. (C) Immunostaining for CD3 (APAAP)); most of the medium sized cells are CD3 positive, whereas the HRS cells (arrows) are CD3 negative. (D) Immunostaining for Epstein-Barr virus encoded latent membrane protein 1 (EBV-LMP-1) (APAAP); the HRS cells are positive for the EBV-LMP-1. (E) Immunostaining for CD20 (APAAP); HRS and some small lymphocytes express CD20. (F) Immunostaining for BSAP (APAAP); HRS and some small lymphocytes are B cell specific activator protein (BSAP) positive.
