Table 2

Summary of results positive for Entamoeba histolytica

	Specimen type	Techlab ELISA	Artus LC-PCR	PCR-SHELA	Storage before DNA extraction
ELISA, enzyme linked immunosorbent assay; LC, Lightcycler; N/A, not available; PCR, polymerase chain reaction; SHELA, solution hybridisation enzyme linked immunoassay.
1	Stool	Negative	Positive	Mixed E histolytica and E dispar	Formol ether concentrate 4°C
2	Stool	Positive	Negative	E dispar	Frozen −20 °C
3	Stool	Positive	Positive	Negative	Frozen −20°C
4	Stool	Positive	Negative	Mixed E histolytica and E dispar	Formol ether concentrate 4°C
5	Stool	Positive	Negative	E histolytica	Formol ether concentrate 4°C
6	Liver pus	N/A	Positive	E histolytica	Frozen −20°C
7	Liver pus	N/A	Positive	E histolytica	Frozen −20°C
8	Liver pus	N/A	Positive	Mixed E histolytica and E dispar	Frozen −20°C
9	Liver pus	N/A	Positive	E histolytica	Frozen −20°C
10	Liver pus	Positive	Positive	E histolytica	Fresh 4°C
11	Stool	Positive	Positive	E histolytica	Frozen −20°C
12	Stool	Positive	Positive	E histolytica	Frozen −20°C
13	Stool	Positive	Positive	E histolytica	Frozen −20°C
14	Stool	Positive	Positive	E histolytica	Formol ether concentrate 4°C
15	Stool	Positive	Positive	E histolytica	Formol ether concentrate 4°C

Comparison of PCR and antigen detection methods for diagnosis of Entamoeba histolytica infection

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