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J Clin Pathol 56:261-267 doi:10.1136/jcp.56.4.261
  • Review

Best Practice No 170

Table 2

Suggested protocols for semen collection and World Health Organisation (WHO) normal values

Collection of a semen sample
Abstain for two to three days
Produce the sample by masturbation, not by coitus interruptus
Do not use condoms for collection of the sample or lubricant jellies
Use a wide mouth sterile plastic specimen pot to collect the sample
Mark the container clearly with the name, date and time of production, and the date of the immediate preceding ejaculation by coitus or masturbation
Deliver to the laboratory as quickly as possible
Protect the sample from extremes of temperature (<15°C or >38°C) during transport—do not keep at body temperature
WHO values 21 for “normal” semen analysis include
Volume 2 ml or more
Concentration, 20 million/ml or more
Motility, 50% progressive motility or more
Morphology, 15% or more normal forms
White blood cells, <1 million/ml

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