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J Clin Pathol 55:699-703 doi:10.1136/jcp.55.9.699
  • Original article

Silent haemoglobin variants and determination of HbA1c with the HPLC Bio-Rad Variant II

Table 1

HbA1c results in patients with haemoglobin (Hb) variants

HbA1c
Haemoglobin variant Variant II HA-8140 DCA 2000 Fructosamine Fasting blood glucose
Variant II, HPLC variant II (Bio-Rad); HA-8140, HPLC high-auto A1c HA-8140 (Menarini); DCA 2000, immunoagglutination DCA 2000 (Bayer); Abnormal sep, abnormal separation; variant Hb, variant haemoglobin.
HPLC, high performance liquid chromatography.
Non-diabetic reference range 4.7–6% 4.5–5.7% 4.5–5.7% <285 μmol/l <6.1 mmol/l
Hb Graz 1 No result Abnormal sep 5.9 466 11.8
Hb Graz 2 No result Abnormal sep 5.2 334 10.6
Hb Sherwood Forest No result Abnormal sep 4.6 238 5.5
Hb D 4.8 9.1 (variant Hb) 5.7 302 6.5
Hb O Padova 1 4.9 6.8 (variant Hb) 4.5 251 6.0
Hb O Padova 2 9.5 10 (variant Hb) 8.8 338 5.2

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