Table 1
An investigation into the effect of pH on serum α1 antitrypsin (AAT) concentrations
| BPL concentration (%) | Base, acid, or buffer added to 2 ml serum aliquots | AAT concentration on day 4 (g/l) | pH on day 5 |
|---|---|---|---|
| Buffer was added before and NaOH was added after β propiolactone (BPL). | |||
| *46 mg Bis-tris+17.9 mg NaCl. | |||
| 0 | Nil | 1.27 | 7.59 |
| 0 | 10 μl 6M HCl | 0.99 | 5.67 |
| 0 | 20 μl 6M HCl | 0.34 | 4.38 |
| 0.25 | Nil | 0.8 | 5.46 |
| 0.5 | Nil | <0.29 | 4.74 |
| 0.5 | Buffer* | 0.81 | 6.33 |
| 1 | Nil | <0.29 | 4.47 |
| 1 | Buffer* | 0.7 | 5.72 |
| 1 | 220 μl 1M NaOH | 0.62 | 7.7 |
