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J Clin Pathol 55:940-945 doi:10.1136/jcp.55.12.940
  • Original article

Detection of cyclin D1 in B cell lymphoproliferative disorders by flow cytometry

Table 3

Correlation between cyclin D1 by flow cytometry, FISH, and immunohistochemistry

Diagnosis Flow cytometry FISH Immunohistochemistry
The 5D4 clone anti-cyclin D1 antibody (Immunotech) was used for flow cytometry; the DCS-6 clone anti-cyclin D1 antibody (Dako) was used for immunohistochemistry.
CLL, chronic lymphocytic leukaemia; FISH, fluorescence in situ hybridisation; MCL, mantle cell lymphoma.
MCL Positive Positive Positive
MCL Positive Positive Positive
MCL Negative Positive Positive
MCL Negative Positive Positive
CD5+B-NHL Positive Negative Negative
CLL Negative Negative Negative
CLL Negative Negative Negative
CLL Negative Negative Negative
Atypical CLL Negative Negative Negative

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