Table 2
Comparison of laboratory parameters of API20C and PCR sequencing techniques
| Laboratory parameter | PCR sequence identification | API20C |
|---|---|---|
| Time to result | ||
| Culture time | – | 72 hours |
| DNA extraction | 1 hour | |
| PCR amplification | 3 hours | |
| Agarose gel electrophoresis | 15 minutes | |
| Clean up of PCR product | — | |
| Sequencing protocol | 16 hours (overnight) | |
| Total time | 20 hours 15 minutes | 72 hours5 |
| Relative cost/isolate | High | Moderate |
| Ease of use | Complex | Simple |
| Repeatability | Excellent | Good |
| Subjectivity | Low | Moderate |
| Requirements for complex equipment | High | Low |
| Applicability to routine diagnostic laboratory | Poor | Good |
