Table 2
Southern blot analysis of Ig/TCR genes in patients with discordant molecular results (n = 2) or inconclusive histopathological diagnosis (n = 7)
| Case | Category | Combined histomorphology and immunohistochemistry | SB analysis of Ig/TCR genes | PCR analysis of IGH genes (FR3/FR2/FR1) | Follow up analysis and clinical data |
|---|---|---|---|---|---|
| AILD, angioimmunoblastic lymphoproliferative disease; B-NHL, B cell non-Hodgkin's lymphoma; DLCL, diffuse large B cell lymphoma; FL, follicular lymphoma; FR, framework region; HD, Hodgkin's disease; IGH, immunoglobulin heavy chain; LL, lymphocytic leukaemia; TCR, T cell receptor; TdT, terminal deoxy nucleotidyl transferase; T-NHL, T cell non-Hodgkin's lymphoma. | |||||
| 767 | Discordant | DLCL | Non-clonal | Polyclonal | Diagnosis of B-NHL also based on earlier biopsy; treated for B-NHL |
| 95-040 | Discordant | FL (revised; initially reactive) | Non-clonal | Polyclonal | Unknown |
| 759 | Inconclusive | Suspected B-NHL (LL) | Clonal | Polyclonal | Unknown |
| (IGH/IGK) | |||||
| 766 | Inconclusive | Suspected acute lymphoblastic leukaemia | Clonal | Polyclonal | Not treated, despite presence of TdT+ cells in lymph node |
| (IGH/IGK/IGL) | |||||
| 818 | Inconclusive | Suspected B-NHL | Clonal | Polyclonal | Diagnosis of DLCL on next biopsy; relapse of DLCL in neck 4 years later |
| (IGH/TCRB) | |||||
| 94-105 | Inconclusive | Differential diagnosis, B-NHL/HD | Clonal | Clonal | Remission after treatment for T rich B-NHL; |
| (IGH/IGK) | (FR2/FR1) | 2 years later presentation of lymphocyte rich nodular HD; B-NHL perceived as transformed phase of underlying HD | |||
| 95-056 | Inconclusive | Differential diagnosis, HD/B-NHL | Non-clonal | Polyclonal | Treated for lymphocyte rich nodular HD; follow up 4 years later: no localisation of HD in lymph node |
| 95-054 | Inconclusive | Reactive, but suspect | Non-clonal | Polyclonal | Treated for nodular sclerotic HD; follow up 1 year later: reactive lymph node |
| 95-036 | Inconclusive | Suspected T-NHL | Non-clonal | ND | Sepsis leading to death; AILD T-NHL at necropsy |
