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J Clin Pathol 54:362-366 doi:10.1136/jcp.54.5.362

Rapid identification of candida species by TaqMan PCR

Table 4

Identification of clinical isolates by candida TaqMan™ assay

Mycology identification TaqMan result Final identification
Mycology identification: identification was based on API 20C and germ tube formation for C albicans.
*Putative identification based on sequencing of the internal transcribed spacer region ITS2 region, germ tube formation, chlamydospore production, and lack of growth at 45°C.
Candida albicans (n = 16) C albicans 14/16 C albicans 14/16
Negative 2/16 C dubliniensis* 2/16
C tropicalis (n = 12) C tropicalis 12/12 C tropicalis 12/12
C parapsilosis (n = 13) C parapsilosis 13/13 C parapsilosis 13/13
C krusei (n = 10) C krusei 10/10 C krusei 10/10
C kefyr (n = 3) C kefyr 3/3 C kefyr 3/3
C glabrata (n = 12) C glabrata 12/12 C glabrata 12/12
Saccharomyces cerevisiae (n = 10) Negative 9/10 S cerevisiae 9/10
C parapsilosis 1/10 S cerevisiae + C parapsilosis 1/10
Cryptococcus neoformans (n = 1) Negative 1/1 Cryptococcus neoformans 1/1
C lusitaniae (n = 1) Negative 1/1 C lusitaniae 1/1
C famata (n = 2) Negative 2/2 C famata 2/2

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