Table 2
Primers and polymerase chain reaction (PCR) conditions
| Primers | Sequences | Genes | Size of anplified DNA | MgCl2 concentration | PCR conditions |
|---|---|---|---|---|---|
| HCMV, human cytomegalovirus; HSV, herpes simplex virus; VZV, varicella zoster virus. | |||||
| HSV-1TK | TkR`-5′tcagttagcctcccccatc | Thymidine kinase | 1130 bp | 1 mM | 95°C 1 min, 55°C 1 min, 72°C 1 min (35 cycles), and 72°C 15 min |
| TkF`-5′atggcttcgtacccctgcc | |||||
| VZV-TK | TkR`-5′aggaagtgttgtcctgaacggc | Thymidine kinase | 1024 bp | 2 mM | 95°C 1 min, 57°C 1 min, 72°C 1 min (35 cycles), and 72°C 15 min |
| TkF`-5′atgtcaacggataaaaccgatgt | |||||
| HCMV | PolF-5′gctatgtttcagatgtcgccgcc | DNA polymerase | 170 bp | 1.5 mM | 95°C 1 min, 62°C 1 min, 72°C 1 min (35 cycles), and 72°C 15 min |
| DNApol | PolR-5′cccacctcgggctc aaacac | ||||
