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J Clin Pathol 53:645-646 doi:10.1136/jcp.53.8.645-a

Some problems found in HIV-1 RNA quantification

Table 1

Amplicor TM HIV-1 monitor kit

Case Ver. 1.0 Ver. 1.0 (+) Ver. 1.5
Test results of each case are summarised.
Measurements with Amplicor HIV-1 monitor test kits Ver. 1.0, 1.0 plus, and 1.5 were performed according to the manufacturer's protocol.
The per cent coefficient of variation on the same samples tested with the Amplicor HIV-1 monitor test kits Ver. 1.0 and 1.0 plus in our laboratory were 19.9% (n = 17) and 31.9% (n = 12), respectively. In cases 2 and 6 the viral load was confirmed with another independent measurement (second result).
Case 1 3.6 × 104 2.7 × 104 2.0 × 104
Case 2 <0.4 × 103 3.7 × 104 <0.4 × 103
<0.4 × 103 2.1 × 104
Case 3 4.2 × 103 1.2 × 104 9.2 × 103
Case 4 1.7 × 103 5.0 × 104 3.5 × 104
Case 5 1.6 × 103 6.1 × 104 3.5 × 104
Case 6 4.6 × 104 <0.4 × 103 0.5 × 103
3.1 × 104 <0.4 × 103

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