Immunohistowax processing, a new fixation and embedding method for light microscopy, which preserves antigen immunoreactivity and morphological structures: visualisation of dendritic cells in peripheral organs

Table 1

Effect of zinc fixation on antigen preservation

		Staining score
Fixation	Dehydration	MHC II	CD11c	B220	Mac-1	CD3	CD4	CD8
Acetone: one bath of 100% acetone for six hours.
Ethanol: graded series of 30%, 50%, 70%, 90%, and 100% of ethanol, 30 minutes in each bath.
–, Negative; +, weakly positive; ++, positive; +++, strongly positive.
MHC, major histocompatibility complex.
	Acetone	+++	+++	+++	+++	–	–	–
Zinc fixative (3 days)	Acetone	+++	+++	+++	+++	++	++	++
Zinc fixative (3 days)	Ethanol	+++	+++	+++	+++	++	++	++

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Immunohistowax processing, a new fixation and embedding method for light microscopy, which preserves antigen immunoreactivity and morphological structures: visualisation of dendritic cells in peripheral organs

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