Immunohistowax processing, a new fixation and embedding method for light microscopy, which preserves antigen immunoreactivity and morphological structures: visualisation of dendritic cells in peripheral organs

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Figure 3

Phenotype and morphology of lung dendritic cells after the inoculation of bacteria. Immunohistowax processed lung sections from mice injected with phosphate buffered saline (A) or live Escherichia coli (B–E) were double stained with anti-CD11c in red and anti-I-E^d in blue. Note the CD11c positive, major histocompatibility complex class II positive cells in purple (B–E). Scale bars, 100 μm (A and B); 50 μm (C–E).

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Immunohistowax processing, a new fixation and embedding method for light microscopy, which preserves antigen immunoreactivity and morphological structures: visualisation of dendritic cells in peripheral organs

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