Immunohistowax processing, a new fixation and embedding method for light microscopy, which preserves antigen immunoreactivity and morphological structures: visualisation of dendritic cells in peripheral organs

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Figure 2

Histopathology of the lung after Escherichia coli injection. Lungs from Balb/c mice injected with phosphate buffered saline (A and C) or E coli (B, D, and E) one hour previously were fixed, dehydrated, and embedded according to the Immunohistowax processing protocol and stained with haemalun-eosin. Note the infiltration of neutrophils in alveoli (D) and in arterioles (E). Scale bars, 250 μm (A and B); 25 μm (C–E).

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Immunohistowax processing, a new fixation and embedding method for light microscopy, which preserves antigen immunoreactivity and morphological structures: visualisation of dendritic cells in peripheral organs

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