Table 1
Cellular distribution pattern of Chlamydia pneumoniae components at different times after infection in the lungs of mice infected intranasally with C pneumoniae
| C pneumoniae antigens | ||||||
|---|---|---|---|---|---|---|
| Type of cells | Days post infection | C pneumoniae DNA and 16S rRNA | Membrane protein | Lipopoly-saccharide | Heat shock protein 60 | Staining pattern |
| The results are the combined results of three mice at two days, three mice at seven days, and four mice at 21 days after infection. All mice at the same time point after infection showed a similar staining pattern. | ||||||
| Chlamydia pneumoniae DNA and 16S rRNA were determined by in situ hybridisation; the staining pattern was always inclusion-like. | ||||||
| Chlamydia pneumoniae antigens were determined by immunocytochemistry. | ||||||
| Number of positive cells graded: –, none; ±, <5/section; +, 5–25/section; ++, 26–100/section; +++, >100/section; ND, not done. | ||||||
| Staining patern: G, granular; I, inclusion-like; NA, not applicable. | ||||||
| Bronchus and alveolar epithelium | 2 | ++ | ++ | ++ | + | I |
| 7 | ND | + | + | + | I | |
| 21 | ± | – | – | – | NA | |
| Alveolar macrophages | 2 | + | ++ | ++ | ++ | G |
| 7 | ND | + | +++ | ++ | G | |
| 21 | – | + | + | ± | G | |
| Infiltrate cells | 2 | ++ | ± | ++ | – | I and G |
| 7 | ND | ++ | ++ | + | I and G | |
| 21 | ± | +/– | – | + | G | |
| Macrophages in bronchus associated lymphoid tissue | 2 | – | – | – | – | NA |
| 7 | ND | ± | ± | ± | G | |
| 21 | ± | + | + | + | G | |
