Table 2
Binding of annexin V, activated complement, and apolipoprotein H to apoptotic cells
| Annexin V (–) Serum | Annexin V (+) Serum | |||
|---|---|---|---|---|
| (–) | (+) | (–) | (+) | |
| Jurkat cells were incubated with 25 μM etoposide overnight. Cells were then analysed for binding of annexin V, activated complement, or apolipoprotein H via fluorecent activated cell sorter (FACS) analysis. | ||||
| Serum concentration was 2.5%. | ||||
| Results are mean fluorescent intensity of five experiments. | ||||
| Anti-apoH was biotinylated rabbit polyclonal 34. | ||||
| Control | 12 | 13 | 47 | 55 |
| Anti-apoH | 13 | 16 | 66 | 328 |
| Anti-C3-9 | 10 | 13 | 64 | 584 |
