The determination of urinary 17-ketosteroids by an improved Zimmermann reaction
- Institute of Clinical Pathology and Medical Research, Department of Public Health, Sydney, Australia 1
Improvements in the Zimmermann reaction are effected by using methanol as a solvent for the reagents employed in the reaction in place of ethanol or aqueous ethanol. The advantages are as follows. Considerably lower blanks are obtained; extraneous chromogens are negligible at the wavelength of measurement, so that no correction procedure or extraction process is required; high concentrations of potassium hydroxide may be used at an elevated temperature leading to good sensitivity and a considerably shortened reaction time; and finally, the potassium hydroxide is stable for long periods under good storage conditions. A steroid with keto (oxo) groups at positions 3, 11, and 20, but not at 17, failed to give any coloured product.
1 Postal address: Institute of Clinical Pathology and Medical Research, Post Office Box 108, Lidcombe, New South Wales, Australia.