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CCN abstracts

Oral communications
O1 CTGF EXPRESSION IS SENSITIVE TO CHANGES OF THE ACTIN CYTOSKELETON IN ENDOTHELIAL CELLS
S. Muehlich¹, B. Krueger¹, C. D. Garlichs², M. Goppelt-Struebe¹.¹Medical Clinic IV and ²Medical Clinic II, University of Erlangen-Nuernberg, Erlangen, Germany

CTGF has been shown to be upregulated in human umbilical vein endothelial cells (HUVEC) under turbulent flow conditions. This prompted us to investigate the molecular mechanisms that might link changes in cell morphology and CTGF gene expression.

CTGF expression was studied in primary cultures of HUVEC. Manipulation of the actin cytoskeleton revealed a reverse correlation between CTGF expression and the cellular concentration of monomeric G-actin: when the actin cytoskeleton was disrupted by latrunculin, CTGF mRNA and protein synthesis decreased. When monomeric G-actin was reduced by enhanced formation of F-actin stress fibres by yasplakinolide, CTGF synthesis was increased. Similarly, disruption of microtubuli by colchicine and the subsequent increase in F-actin strongly enhanced CTGF synthesis.

To provide direct evidence for the role of G-actin in CTGF expression, . . . [Full text of this article]