Analysis of microsatellite instability in colorectal carcinoma by microfluidic-based chip electrophoresis
Margarete Odenthal 1*, Nora Barta 1, Daniela Lohfink 1, Uta Drebber 1, Falko Schulze 1, Hans Peter Dienes 1 and Stephan E Baldus 2
1 Institute of Pathology, University of, Germany
2 Institute of Pathology, University of Duesseldorf, Germany
* To whom correspondence should be addressed. E-mail: m.odenthal{at}uni-koeln.de.
Accepted 20 June 2008
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Abstract |
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Microsatellite analysis is an important tool in clinical research and molecular diagnostics, because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10-15% of colorectal, gastric or endometrial carcinomas are associated with MSI, which has an impact on clinical prognosis.
The microsatellite loci Bat25, Bat26, D2S123, D5S346, and D17S250, recommended by the Bethesda guidelines, were analysed by microfluidic-based on-chip electrophoresis in 40 cases of colon carcinoma with known MSI status. In all cases, microfluidic separation of the PCR amplicons resulted in highly resolved, distinct patterns of each of the five microsatellite loci. Detection of MSI could be demonstrated by microsatellite loci-associated, well-defined deviations in the electropherogram profiles of tumour and non-tumour material and confirmed the classification of MSI cases performed by conventional technology.
In conclusion, microfluidic chip technology is a simple and reliable approach for MSI detection, which allows label-free and very fast analysis of microsatellite amplicons.
