ORIGINAL ARTICLE

Survivin promoter polymorphism and cervical carcinogenesis

A A Borbély¹, M Murvai², K Szarka¹, J Kónya¹, L Gergely², Z Hernádi³ and G Veress¹

¹ Department of Medical Microbiology, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary
² Tumour Virus Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary
³ Department of Obstetrics and Gynecology, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary

Correspondence to:
Correspondence to:
Dr G Veress
Department of Medical Microbiology, Medical and Health Science Centre, University of Debrecen, H-4012 Debrecen, POB 17, Hungary;veregy{at}dote.hu

Background: Survivin, a novel member of the inhibitor of apoptosis family, plays an important role in cell cycle regulation. A common polymorphism at the survivin gene promoter (G/C at position 31) was shown to be correlated with survivin gene expression in cancer cell lines.

Aim: To investigate whether this polymorphism could be involved in the development of human papillomavirus (HPV)-associated cervical carcinoma.

Methods: Survivin promoter polymorphism was detected in patients with cervical cancer, in patients with equivocal cytological atypia and in a control population using polymerase chain reaction (PCR-restriction fragment length polymorphism (RFLP) and PCR-single strand conformation polymorphism analysis. HPV was typed in patients with cervical cancer and cytological atypia using PCR-RFLP.

Results: No statistically significant differences were found in the genotype distributions of the survivin promoter variants among our study groups.

Conclusions: The survivin promoter polymorphism at position 31 may not represent an increased risk for the development of cervical cancer, at least in the population studied here.

Abbreviations: ASC, atypical squamous cells; CDE, cycle-dependent element; CHR, cycle homology region; HPV, human papillomavirus; PCR, polymerase chain reaction; RFLP, restriction fragment length polymorphism; SSCP, single-strand conformation polymorphism

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