Journal of Clinical Pathology 2006;59:645-654
ORIGINAL ARTICLE
Comparative investigations of T cell receptor 
gene rearrangements in frozen and formalin-fixed paraffin wax-embedded tissues by capillary electrophoresis
University Institute of Pathology, Århus University Hospital, Århus Hospital, Århus, Denmark
Correspondence to:
Correspondence to:
Dr Mariann Christensen
University Institute of Pathology, Århus University Hospital, Tage-Hansens Gade 2, DK-8000 Århus, Denmark; E-mail: lab10mc{at}as.aaa.dk
Aim: To compare clonal T cell receptor 
(TCR) gene rearrangements in frozen and formalin-fixed paraffin wax-embedded (FFPE) tissue, using capillary electrophoresis for use in diagnostics, as T cell lymphomas may be difficult to diagnose by conventional methods.
Methods: The DNA for PCR was extracted from frozen and FFPE tissue, cell lines and blood. PCR primers V1-8, V9, V10 or V11 (5' end labelled) combined with a mixture of JP1/JP/JP2/J2 (unlabelled) were used. Monoclonal cases were sequenced and clonality, reproducibility, sensitivity and specificity analyses were carried out.
Results: In all cases the molecular test was found to be in agreement with the histological diagnosis. Discrepancies were found between frozen and FFPE tissue in 18 of 56 (32%) tests. The method was highly reproducible. The sensitivity was found to be 0.5% for cell lines and 1% for patient specimens and the specificity 100%. The junctional region between the V and J segments was specific for each patient.
Conclusions: Capillary electrophoresis of PCR products from frozen and FFPE tissue is suitable for detecting clonal TCR gene rearrangements. It is important, however, to correlate the results with conventional morphological and immunohistochemical studies.
Abbreviations: FFPE, formalin-fixed and paraffin wax-embedded; TCR, T cell receptor
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
jcp.2005.025809v1
59/6/645 most recent - Submit a response
- Alert me when this article is cited
- Alert me when eLetters are posted
- Alert me if a correction is posted
- Citation Map
Services
- Email this link to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
PubMed
Topic Collections
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
