HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER		Author Keyword(s) Vol Page [Advanced]

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this link to a friend Similar articles in this journal Similar articles in PubMed Add article to my folders Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lau, S K P Articles by Yuen, K-y Search for Related Content PubMed PubMed Citation Articles by Lau, S K P Articles by Yuen, K-y

Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures

¹ Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong
² Department of Clinical Pathology, Tuen Mun Hospital, Hong Kong

Correspondence to:
Dr K-Y Yuen
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong; hkumicro{at}hkucc.hku.hk
ABSTRACT
Using full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.

Abbreviations: rRNA, ribosomal RNA

Keywords: Microseq 500; 16S rDNA gene; anaerobic Gram positive bacilli; blood cultures

This article has been cited by other articles:

				R. P. Rennie, C. Brosnikoff, L. Turnbull, L. B. Reller, S. Mirrett, W. Janda, K. Ristow, and A. Krilcich Multicenter Evaluation of the Vitek 2 Anaerobe and Corynebacterium Identification Card J. Clin. Microbiol., August 1, 2008; 46(8): 2646 - 2651. [Abstract] [Full Text] [PDF]

				E. E. Adderson, J. W. Boudreaux, J. R. Cummings, S. Pounds, D. A. Wilson, G. W. Procop, and R. T. Hayden Identification of Clinical Coryneform Bacterial Isolates: Comparison of Biochemical Methods and Sequence Analysis of 16S rRNA and rpoB Genes J. Clin. Microbiol., March 1, 2008; 46(3): 921 - 927. [Abstract] [Full Text] [PDF]

				P. C Y Woo, L. M W Chung, J. L L Teng, H. Tse, S. S Y Pang, V. Y T Lau, V. W K Wong, K.-l. Kam, S. K P Lau, and K.-Y. Yuen In silico analysis of 16S ribosomal RNA gene sequencing-based methods for identification of medically important anaerobic bacteria J. Clin. Pathol., May 1, 2007; 60(5): 576 - 579. [Abstract] [Full Text] [PDF]

				S. K. P. Lau, J. L. L. Teng, K.-W. Leung, N. K. H. Li, K. H. L. Ng, K.-Y. Chau, T.-L. Que, P. C. Y. Woo, and K.-Y. Yuen Bacteremia Caused by Solobacterium moorei in a Patient with Acute Proctitis and Carcinoma of the Cervix. J. Clin. Microbiol., August 1, 2006; 44(8): 3031 - 3034. [Abstract] [Full Text] [PDF]