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This Article Full Text Full Text (PDF) All Versions of this Article: jcp.2005.031922v1 jcp.2005.031922v2 jcp.2005.031922v3 59/11/1175    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this link to a friend Similar articles in this journal Similar articles in PubMed Add article to my folders Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mueller, S Articles by Stolte, M Search for Related Content PubMed PubMed Citation Articles by Mueller, S Articles by Stolte, M Pubmed/NCBI databases Medline Plus Health Information Eosinophilic Disorders

Eosinophil infiltration and degranulation in oesophageal mucosa from adult patients with eosinophilic oesophagitis: a retrospective and comparative study on pathological biopsy

¹ Institute of Pathology, Klinikum Bayreuth, Bayreuth, Germany
² Department of Pathology, University of Erlangen-Nuremberg, Erlangen, Germany
³ Institute of Pathology, Paracelsus Private Medical University, General Hospital Salzburg, Salzburg, Austria

Correspondence to:
S Mueller
Institute of Pathology, Leipzig University, Liebigstr. 26, D 04103 Leipzig, Germany; mueller.susanna{at}t-online.de Aim: To examine eosinophil infiltration and degranulation in 50 oesophageal biopsy specimens from 30 patients (21 men, 9 women; mean 39 years) with eosinophilic oesophagitis, by haematoxylin and eosin staining and immunohistochemistry.

Methods: Immunohistochemistry was carried out using a monoclonal antibody for human eosinophilic major basic protein (MBP). Eosinophils were counted in three high power fields (x40) and degranulation, as quantified by extracellular MBP immunostaining, was scored on a scale of 1–4. Morphological changes (basal cell hyperplasia, elongation of papillae and dilatation of intercellular spaces) were scored on a 1–4 scale on sections stained with haematoxylin and eosin.

Results: Numbers of intraepithelial eosinophils were significantly higher with MBP immunostaining than with haematoxylin and eosin staining (mean 109.6 v 80.6; p<0.001), whereas numbers of eosinophils were considerably correlated (r = 0.794). Eosinophil degranulation was higher in the distal oesophagus. Additionally, basic morphological changes were markedly associated with eosinophil infiltration. Extracellular deposition of eosinophil-MBP and eosinophil infiltration in subepithelial connective tissue, present in the biopsy specimens, were detected by immunohistochemistry.

Conclusion: Numbers of eosinophils and degranulation are underestimated by haematoxylin and eosin staining. Immunohistochemistry detected up to two times more eosinophils than routine haematoxylin and eosin staining. Moreover, eosinophil-MBP immunoreactivity in extracellular regions indicates the release of toxic eosinophil granule proteins and gives further evidence for a causative role of eosinophils with regard to structural changes in eosinophilic oesophagitis. Immunohistochemistry may serve as a useful diagnostic tool to support the morphological differential diagnosis of eosinophilic oesophagitis and gastro-oesophageal reflux disease.

Abbreviations: ECP, eosinophilic cationic protein; GERD, gastro-oesophageal reflux disease; HPF, high power field; MBP, major basic protein