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SHORT REPORT |
1 The Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
2 The Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611
3 The Pathology Core Facility, Northwestern University Feinberg School of Medicine
4 Department of Preventive Medicine, Robert H Lurie Comprehensive Cancer Center, Chicago, IL 60611, USA
Correspondence to:
Dr V Ananthanarayanan
Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, 680 North Lake Shore Drive, Suite1102, Chicago, IL 60611, USA; viju{at}northwestern.edu
ABSTRACT
Aims: To investigate the problems involved in undertaking immunohistochemistry (IHC) and nuclear morphometry using Bouins fixed prostate biopsies.
Methods: Archival Bouins fixed and formalin fixed, paraffin wax embedded prostatic biopsies were immunostained for three nuclear biomarkers (minichromosome maintenance protein 2 (MCM-2), p27, and Ki-67), one membrane localised biomarker (C-erb-B2), CD34, and
methylacyl-CoA racemase (AMACR). The quality of IHC staining was compared between tissues prepared separately in both fixatives. Feulgen staining was also performed on Bouins fixed tissues to check its suitability for nuclear morphometry.
Results: MCM-2 staining was completely negative in Bouins fixed tissues, whereas p27 showed more background and excess cytoplasmic staining in Bouins fixed versus formalin fixed tissues. C-erb-B2 showed non-specific, strong luminal cell staining in the Bouins fixed tissue. Feulgen staining was also very weak in Bouins fixed tissue. However, Ki-67, AMACR, and CD34 worked equally well in Bouins and formalin fixed tissues.
Conclusions: Bouins fixed tissues may be unsuitable when subsequent IHC and morphometry are contemplated. An awareness of which antibodies are suitable for use in Bouins fixed biopsies is essential.
Abbreviations: AMACR,
methylacyl-CoA racemase; BF, Bouins fixed; IHC, immunohistochemistry; MCM-2, minichromosome maintenance protein 2; NBF, neutral buffered formalin
Keywords: Bouins fixative; immunohistochemistry; prostate
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