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Application of the FICTION technique for the simultaneous detection of immunophenotype and chromosomal abnormalities in routinely fixed, paraffin wax embedded bone marrow trephines

P Kora

R Kuec

¹ Nuffield Department of Clinical Laboratory Sciences, University of Oxford, LRF Immunodiagnostics Unit, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
² Department of Clinical Pathology and Cytology, Merkur University Hospital, 10000, Zagreb, Croatia
³ Institute of Clinical Chemistry, Merkur University Hospital

Correspondence to:
Dr R Ventura
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, LRF Immunodiagnostics Unit, Level 5, Room 5501, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK; roland.ventura{at}ndcls.ox.ac.uk
ABSTRACT
The use of interphase fluorescence in situ hybridisation (FISH) to study cytogenetic abnormalities in routinely fixed paraffin wax embedded tissue has become commonplace over the past decade. However, very few studies have applied FISH to routinely fixed bone marrow trephines (BMTs). This may be because of the acid based decalcification methods that are commonly used during the processing of BMTs, which may adversely affect the suitability of the sample for FISH analysis. For the first time, this report describes the simultaneous application of FISH and immunofluorescent staining (the FICTION technique) to formalin fixed, EDTA decalcified and paraffin wax embedded BMTs. This technique allows the direct correlation of genetic abnormalities to immunophenotype, and therefore will be particularly useful for the identification of genetic abnormalities in specific tumour cells present in BMTs. The application of this to routine clinical practice will assist diagnosis and the detection of minimal residual disease.

Abbreviations: ALL, acute lymphoblastic leukaemia; BMT, bone marrow trephine; CLL, chronic lymphocytic leukaemia; FISH, fluorescence in situ hybridisation; FL, follicular lymphoma; MCL, mantle cell lymphoma

Keywords: FICTION; fluorescence in situ hybridisation; lymphoma; bone marrow trephine

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