© 2004 BMJ Publishing Group Ltd & Association of Clinical Pathologists
SHORT REPORT
The diagnosis of hepatosplenic candidiasis by DNA analysis of tissue biopsy and serum
1 Department of Medical Microbiology, Clinical Sciences Building, Manchester Royal Infirmary
2 NeuTec Pharma plc, Clinical Sciences Building, Manchester Royal Infirmary
Correspondence to:
Correspondence to:
Dr A Kirby
Department of Medical Microbiology, Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, UK; kirbyandrew{at}doctors.org.uk
Hepatosplenic candidiasis is traditionally diagnosed by blood culture, magnetic resonance imaging (MRI), and histological analysis. The limitations of these methods include: low sensitivity (blood culture) and failure to isolate the organism (MRI/histology). This report describes a case of hepatosplenic candidiasis diagnosed by simultaneous polymerase chain reaction (PCR) analysis of liver biopsy and serum. PCR of biopsy and/or serum may offer a sensitive and specific diagnostic test for hepatosplenic candidiasis. Candida species can be identified, which helps guide antifungal chemotherapy, an important aspect in this difficult to treat infection.
Abbreviations: ITS, internal transcribed spacer; MIC, minimum inhibitory concentration; PCR, polymerase chain reaction; Tm, melting temperature
Keywords: hepatosplenic candidiasis; tissue biopsy; serum; diagnosis
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