SHORT REPORT

A comparison of IgG anti-rubella activity in frozen serum stored in primary gel separation tubes or secondary tubes

V Ellis¹, A Charlett² and R Bendall¹

¹ Department of Clinical Microbiology, Royal Cornwall Hospital, Penventinnie Lane, Treliske, Truro, Cornwall TR1 3LQ, UK
² Statistics Unit, Health Protection Agency, Communicable Disease Surveillance Centre, 61 Colindale Avenue, London NW9 5EQ, UK

Correspondence to:
Correspondence to:
Dr R Bendall
Department of Clinical Microbiology, Royal Cornwall Hospital, Penventinnie Lane, Treliske, Truro, Cornwall TR1 3LQ, UK; richard.bendall{at}cornwall.nhs.uk

Aims: To determine the suitability of primary gel separation tubes for the storage of frozen sera intended for serological testing.

Methods: Blood samples from 102 patients were collected into gel separation tubes. The sera from these samples were split between the primary gel separation tubes and conventional plastic storage tubes and frozen. A year later, the tubes were thawed and anti-rubella IgG concentrations were compared for the serum pairs using the Wilcoxon signed rank test.

Results: No significant difference was detected between the two storage methods.

Conclusions: Frozen storage of serum samples in primary gel separation tubes is a practical alternative to storing separated sera in secondary containers. Adopting this practice has advantages for laboratories in reducing specimen handling and reducing errors in labelling stored samples.

Keywords: Serum; gel separation; frozen storage; antibodies

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Wasserman, E. E., Nelson, K., Rose, N. R., Eaton, W., Pillion, J. P., Seaberg, E., Talor, M. V., Burek, L., Duggan, A., Yolken, R. H. (2008). Maternal Thyroid Autoantibodies during the Third Trimester and Hearing Deficits in Children: An Epidemiologic Assessment. Am J Epidemiol 167: 701-710 [Abstract] [Full Text]  
• Twomey, P J, Viljoen, A (2004). Limitations of the Wilcoxon matched pairs signed ranks test for comparison studies. J. Clin. Pathol. 57: 783-783 [Full Text]