HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER		Author Keyword(s) Vol Page [Advanced]

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this link to a friend Similar articles in this journal Similar articles in PubMed Add article to my folders Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yea, S S Articles by Paik, K-H Search for Related Content PubMed PubMed Citation Articles by Yea, S S Articles by Paik, K-H Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Substance via MeSH Medline Plus Health Information Stomach Disorders

Association between TNF- promoter polymorphism and Helicobacter pylori cagA subtype infection

The Paik-Inje Memorial Institute for Biomedical Science, Inje University, Pusan 614–735, Korea
Department of Pathology, College of Medicine, Inje University, Paik Hospital, Pusan 614–735, Korea
Department of Internal Medicine, College of Medicine, Inje University, Paik Hospital, Pusan 614–735, Korea

Dr Yang pathyang{at}ijnc.inje.ac.kr Aims—To assess the importance of tumour necrosis factor (TNF-) promoter polymorphism in relation to infection with the cytotoxin associated gene A (cagA) subtype of Helicobacter pylori within a dyspeptic Korean population.

Methods—Eighty three patients with gastric disease and 113 healthy controls were studied. The DNA from gastric biopsy specimens was analysed by H pylori specific and cagA specific polymerase chain reaction (PCR). To characterise TNF- polymorphism at positions -308 and -238, PCR based restriction fragment length polymorphism analysis was performed.

Results—Helicobacter pylori infection was closely correlated with G to A transition at position -308 of the TNF- promoter when compared with healthy controls (odds ratio (OR), 2.912; 95% confidence interval (CI), 1.082 to 7.836; p = 0.034). Although TNF- -308 polymorphism in patients with H pylori was not significantly different from that in patients without H pylori, the -308A polymorphism was strongly associated with H pylori cagA subtype infection when compared with the polymorphism in cagA negative H pylori infection (OR, 8.757; 95% CI, 1.413 to 54.262; p = 0.019) and healthy controls (OR, 3.683; 95% CI, 1.343 to 10.101; p = 0.011). G to A genetic change at position -238 of the TNF- gene was not significantly associated with H pylori cagA subtype infection. In addition, genetic polymorphisms at both sites of the TNF- promoter in patients with H pylori infection did not correlate with the severity of disease.

Conclusion—TNF- -308A polymorphism was significantly related to infection with the H pylori cagA subtype in Korean patients with gastric disease.

Key Words: Helicobacter pylori • cagA • tumour necrosis factor • polymorphism

This article has been cited by other articles:

				J. G. Kusters, A. H. M. van Vliet, and E. J. Kuipers Pathogenesis of Helicobacter pylori Infection Clin. Microbiol. Rev., July 1, 2006; 19(3): 449 - 490. [Abstract] [Full Text] [PDF]

				F Navaglia, D Basso, C-F Zambon, E Ponzano, L Caenazzo, N Gallo, A Falda, C Belluco, P Fogar, E Greco, et al. Interleukin 12 gene polymorphisms enhance gastric cancer risk in H pylori infected individuals J. Med. Genet., June 1, 2005; 42(6): 503 - 510. [Full Text] [PDF]

				O. Handa, Y. Naito, T. Takagi, M. Shimozawa, S. Kokura, N. Yoshida, H. Matsui, G. Cepinskas, P. R. Kvietys, and T. Yoshikawa Tumor Necrosis Factor-{alpha}-Induced Cytokine-Induced Neutrophil Chemoattractant-1 (CINC-1) Production by Rat Gastric Epithelial Cells: Role of Reactive Oxygen Species and Nuclear Factor-{kappa}B J. Pharmacol. Exp. Ther., May 1, 2004; 309(2): 670 - 676. [Abstract] [Full Text] [PDF]

				N. Katsuda, N. Hamajima, A. Tamakoshi, K. Wakai, K. Matsuo, T. Saito, K. Tajima, and S. Tominaga Helicobacter Pylori Seropositivity and the Myeloperoxidase G-463A Polymorphism in Combination with Interleukin-1B C-31T in Japanese Health Checkup Examinees Jpn. J. Clin. Oncol., April 1, 2003; 33(4): 192 - 197. [Abstract] [Full Text] [PDF]

				M. J. Blaser Polymorphic Bacteria Persisting in Polymorphic Hosts: Assessing Helicobacter pylori-Related Risks for Gastric Cancer J Natl Cancer Inst, November 20, 2002; 94(22): 1662 - 1663. [Full Text] [PDF]

				F. Wieser, G. Fabjani, C. Tempfer, C. Schneeberger, R. Zeillinger, J. C. Huber, and R. Wenzl Tumor Necrosis Factor-{alpha} Promotor Polymorphisms and Endometriosis Reproductive Sciences, September 1, 2002; 9(5): 313 - 318. [Abstract] [PDF]

				P J van Diest and H Holzel Cervical cancer J. Clin. Pathol., April 1, 2002; 55(4): 241 - 242. [Full Text] [PDF]