JCP

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER
[Advanced]

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this link to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Add article to my folders
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Buerger, H
Right arrow Articles by Brandt, B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buerger, H
Right arrow Articles by Brandt, B
Right arrowPubmed/NCBI databases
*UniSTS
Medline Plus Health Information
*Breast Cancer
J Clin Pathol 2001; 54:836-840
© 2001 Journal of Clinical Pathology

Genetic characterisation of invasive breast cancer: a comparison of CGH and PCR based multiplex microsatellite analysis

H Buerger1, H Schmidt2, A Beckmann2, K S Zänker3, W Boecker1, B Brandt2

1 Gerhard-Domagk-Institute of Pathology, University of Münster, 48149 Münster, Germany
2 Institute of Clinical Chemistry and Laboratory Medicine, University of Münster
3 Institute of Immunology, University of Witten/Herdecke, 48543 Witten, Germany

Dr Brandt, Institute of Clinical Chemistry and Laboratory Medicine, Westfälische Wilhelmsuniversität Münster, Albert-Schweitzer-Str. 33, 48149 Münster, Germany brandt{at}uni-muenster.de Aims—Comparative genomic hybridisation (CGH) is a reliable tool to gain an overview of all unbalanced chromosomal alterations within a tumour. Nevertheless, the high numbers of tumour cells required and the comparatively low resolution are drawbacks of this technique. Polymerase chain reaction (PCR) based multiplex microsatellite analysis represents a semi-automated, highly reproducible method, which requires small amounts of tumour cells. This is a comparative study of CGH and microsatellite analysis.

Methods—Eighty one samples of invasive breast cancer were investigated by two sensitive multiplex PCRs containing three microsatellites each of six markers (D6S261, D11S907, D6S300, D11S927, D8S272, and D11S925), and two additional microsatellite markers located within intron 1 of the epidermal growth factor recepter gene (egfr) and p53 (p53CA).

Results—At least one example of loss of heterozygosity was detectable in all breast cancer tissues. However, the overall rate of accordance between the two methods tested was only 61%. An increasing rate of the number of genetic alterations in each case was mirrored by a constantly increasing fractional allelic loss index.

Conclusions—PCR based multiplex microsatellite analysis using this panel of eight microsatellite markers not only enables the characterisation of cells that have malignant potential in a high frequency of patients with breast cancer, but can also give an estimate of the degree of genetic progression.

Key Words: breast cancer • comparative genomic hybridisation • microsatellites • epidermal growth factor • p53




This article has been cited by other articles:


Home page
J. Clin. Pathol.Home page
M M Weiss, E J Kuipers, S G M Meuwissen, P J van Diest, and G A Meijer
Comparative genomic hybridisation as a supportive tool in diagnostic pathology
J. Clin. Pathol., July 1, 2003; 56(7): 522 - 527.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
N. Tidow, A. Boecker, H. Schmidt, K. Agelopoulos, W. Boecker, H. Buerger, and B. Brandt
Distinct Amplification of an Untranslated Regulatory Sequence in the egfr Gene Contributes to Early Steps in Breast Cancer Development
Cancer Res., March 15, 2003; 63(6): 1172 - 1178.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER
Journal of Clinical Pathology Molecular Pathology
Terms and conditions relating to subscriptions purchased online  ¦  Website terms and conditions  ¦  Privacy policy
Copyright © 2001 by the BMJ Publishing Group Ltd & Association of Clinical Pathologists.