JCP -- Pajak et al. 53 (7): 518 Table 2

Table 2 Antigens successfully detected on Immunohistowax processed mouse tissue sections Antigens Clones Specificity Dilution Sources

CD2 12–15 Cell adhesion molecule 10–20 µg/ml Southern Biotechnology Associates, Inc CD3 7D6 T cells 20–30 µg/ml Coulie and colleagues¹⁴ CD4 RM4–5 T helper cells 20 µg/ml Pharmingen CD8 53–6.7 T cells 20 µg/ml Pharmingen CD90/Thy 1.2 53–2.1 T cells 10–20 µg/ml Pharmingen TCR ${alpha}$ ß H57–597 T cells (TCR) 20–30 µg/ml Pharmingen TCR ${gamma}$ ${delta}$ 13D5 T cells (TCR) 20–30 µg/ml Peterman and colleagues¹⁵ TCR Vß8 F23.2 T cells (TCR) 20 µg/ml ATCC TCR Vß6 44.22.1 T cells (TCR) 20 µg/ml Payne and colleagues¹⁶ B220/CD45R RA3–6B2 B cells 5–10 µg/ml Pharmingen IgD LoMD6 B cells 5–10 µg/ml Lefebvre and colleagues¹⁷ IgM LoMM9 B cells 5–10 µg/ml Lefebvre and colleagues¹⁷ MHC-II 14.4.4 B cells, DC, M ${phi}$ 5–10 µg/ml ATCC CD11c N418 Mainly DC 5–10 µg/ml Metlay and colleagues¹⁸ CD11c HL3 Mainly DC 5–10 µg/ml Pharmingen CD11b/Mac-1 M1/70 M ${Phi}$ 5–10 µg/ml Pharmingen NK 5E6 NK cells 10–20 µg/ml Pharmingen Pan-NK DX5 NK cells 10–20 µg/ml Pharmingen Ly-6G/GR1 RB6–8C5 Granulocytes 10–20 µg/ml Pharmingen PMN antigen 7/4 Neutrophils 10–20 µg/ml Caltag CD40 3/23 B cells, DC, M ${phi}$ 10–20 µg/ml Hasbold and colleagues¹⁹ Intracytoplasmic granules 2A1 Mature DC 5–10 µg/ml Inaba and colleagues²⁰ Intracytoplasmic granules M342 Mature DC Undiluted supernatant Agger and colleagues²¹ DEC-205 NLDC-145 Mature DC 20–30 µg/ml Kraal and colleagues²² CD35 8C12 CR1 (Follicular DC) 5–10 µg/ml Pharmingen CD86/B7.2 GL1 Activated B cells, DC, M ${phi}$ 10–20 µg/ml Hathcock and colleagues²³ CD25 7D4 Activated T cells, B cells, DC 10–20 µg/ml Pharmingen Ly-77 GL7 Activated T cells, B cells 10–20 µg/ml Pharmingen CD44 1M7 Activated T cells 10–20 µg/ml Pharmingen CD69 H1.2F3 Activated T cells 10–20 µg/ml Pharmingen Phosphotyrosin 4G10 Activated cells 20–30µg/ml Upstate Biotechnology Incorporation OX40 mOX40L–hIgG1* Activated T cells 20–30µg/ml Cantab Pharmaceutical Research Ltd VCAM-1 M/K-2 Adhesion molecule 10–20 µg/ml R&D Systems Anti-Ars Ig Ars-BSA° Ars specific B cells 20 µg/ml IL-2 S4B6 Cytokine 20–30 µg/ml Pharmingen IL-4 11B11 Cytokine 20–30 µg/ml Pharmingen INF- ${gamma}$ Db1 Cytokine 20–30 µg/ml Paineau and colleagues²⁴ IL-10 JESS-2A5 Cytokine 20–30 µg/ml Pharmingen

*Fusion protein; °synthesised hapten carrier protein. Ars, arsenate; BSA, bovine serum albumin; DC, dendritic cell; h, human; IFN- ${gamma}$ , interferon ${gamma}$ ; Ig, immunoglobulin; IL, interleukin; L, ligand; MHC, major histocompatibility complex; M ${Phi}$ , macrophage; NK, natural killer; PMN, polymorphonuclear cell; TCR, T cell receptor; VCAM, vascular cell adhesion molecule.