|
|
Staining score
|
| Fixation |
Dehydration |
MHC II |
CD11c |
B220 |
Mac-1 |
CD3 |
CD4 |
CD8 |
|
|
Acetone |
+++ |
+++ |
+++ |
+++ |
– |
– |
– |
| Zinc fixative (3 days) |
Acetone |
+++ |
+++ |
+++ |
+++ |
++ |
++ |
++ |
| Zinc fixative (3 days) |
Ethanol |
+++ |
+++ |
+++ |
+++ |
++ |
++ |
++ |
|
| Acetone: one bath of 100% acetone for six hours. |
| Ethanol: graded series of 30%, 50%, 70%, 90%, and 100% of ethanol, 30 minutes in each bath. |
| –, Negative; +, weakly positive; ++, positive; +++, strongly positive. |
| MHC, major histocompatibility complex. |